玻切二期术后低视力人群的护理策略

目的对玻切二期术后低视力人群采取特殊的护理策略,来平复病人的负面情绪,提高治疗满意度。方法对2019年11月6日至2020年11月6日在我院接受玻璃体切割二次手术的96名低视力患者进行特殊护理干预,然后进行回顾性分析。结果在结合实际病情分析采取的特殊护理策略干预下,病人术后情绪稳定,未出现不良投诉事件,满意度较高。结论对玻切二期术后低视力人群采取特殊的护理干预策略十分必要。     

对分课堂在《中医诊断学》教学中的应用及探索

探讨对分课堂在《中医诊断学》教学中的应用。将对分课堂授课模式引入《中医诊断学》教学过程中，通过学生的课堂表现及课后对学生的问卷调查，评价对分课堂教学模式对《中医诊断学》教学效果的影响。学生普遍认可对分课堂教学模式，学生课堂活跃程度提高、主观能动性增加，自主学习能力增加，学习效率提高。通过对分课堂教学，有助于提高学生对《中医诊断学》的学习热情，提高学习成绩，提升教学质量。具有一定的推广意义。     

基础医学综合实验与实验设计课程教学改革实践

本着以课程为中心的原则，研究组在基础医学综合实验课程建设、运行方式、教学方法改革等方面积极探索；以联系疾病、联系应用为导向，广泛开展基于疾病动物模型制备及干预过程的实验教学；增加"创新性"实验项目，确保课程内容体现出前沿性和时代性；以生物医学前沿方法训练引领课程建设，着力培养学员的学习能力、实践能力和创新能力。此外，在优化以实践应用、创新性为导向的课程内容建设的基础上，打造虚拟仿真实验项目，尝试线上线下混合式教学，全方位提升学员的实践运用能力。     

The effect of moderate-intensity exercise on nightly variability in objectively measured sleep parameters among older women

Objective/Background: Exercise training has been demonstrated to beneficially influence mean-level measures of sleep; however, few studies have examined the impact of an exercise intervention on night-to-night variability in sleep. This study investigated whether four months of moderate-intensity exercise impacted night-to-night variability in sleep among older women. Methods: Participants (n = 49) were randomized to one of two moderate-intensity walking programs with different doses of energy expenditure: low-dose (n = 23: 8 kcal/kg of body weight per week) or high-dose (n = 26: 14 kcal/kg of body weight per week). Sleep parameters were assessed objectively via actigraphy at baseline, mid- (2 months), and postintervention (4 months). Nightly variability in each of the sleep parameters was calculated using the seven-day standard deviation (SD) and a coefficient of variation (SD/mean x 100%). Cardiorespiratory fitness (VO_2peak) was measured at baseline and postintervention using a graded treadmill test. Results: Both measures of nightly variability demonstrated a borderline to significantly lower amount of night-to-night variability in wake time after sleep onset (WASO) and number of awakenings at postintervention in comparison to baseline (p ≤ 0.05). Higher VO_2peak levels at baseline were associated with less time in bed and lower total sleep time variability throughout the exercise intervention (p < 0.05). Conclusion: Overall, participation in moderate-intensity exercise was observed to reduce the amount of nightly variability for WASO and number of awakenings over time in older women.     

胸腔镜下记忆合金环抱器内固定治疗多发肋骨骨折

目的 观察胸腔镜下记忆合金环抱器内固定术治疗多发肋骨骨折的效果。方法 回顾性分析2006年9月至2014年12月采用胸腔镜下记忆合金环抱器内固定治疗多发肋骨骨折患者34例临床资料,与同期采用传统方法(非手术治疗)治疗患者24例进行比较,观察两组胸痛缓解时间、胸引管引流量、呼吸机支持时间、住院时间及并发症发生率的情况。结果 胸腔镜组的胸痛时间、胸引管引流量、呼吸机支持时间、住院时间及并发症发生率均较优于传统方法组,差异均有统计学意义(P<0.05)。结论 胸腔镜下记忆合金环抱器内固定治疗多发肋骨骨折创伤小,患者恢复快,能明显改善患者生活质量。     

应用hMeDIP-chip技术分析尿毒症患者基因组5-羟甲基胞嘧啶状态

目的 通过hMeDIP-chip技术,在全基因组范围内分析尿毒症患者的5-羟甲基胞嘧啶(5-hmC)状态.方法 采用hMeDIP-chip技术筛选出5-hmC水平差异显著的目的基因,并用QRT-PCR验证hMeDIP-chip结果可靠性.结果 实验筛选得到3个目的基因:HMGCR、THBD、STAT3,其结果与hMeDIP-chip结果相符,证明hMeDIP-chip结果可靠.结论 尿毒症患者DNA 5-hmC状态有很大变化,这些变化或许可以进一步揭示尿毒症的发病机制,5-hmC或可作为尿毒症的潜在生物标志物和治疗靶点.     

MHC捕获测序探寻慢性肾功能衰竭的致病基因

目的 研究慢性肾功能衰竭患者人类白细胞抗原(HLA)基因,以获得慢性肾功能衰竭的致病突变.方法 使用新的MHC捕获技术结合新一代高通量测序技术对15例慢性肾功能衰竭患者和15名健康对照的HLA区域基因进行捕获测序.结果 慢性肾功能衰竭的致病与HLA-A* 02∶01∶01、B*15∶01∶01和DRB1* 09∶01∶02关联,且这三个基因相互独立与慢性肾功能衰竭关联.结论 HLA-A*02∶01∶01、B* 15∶01∶01和DRB1* 09∶01∶02与慢性肾功能衰竭相关.采用MHC基因捕获技术结合下一代高通量测序技术研究慢性肾功能衰竭的基因易感性是可行的.     

Effect of high volume hemofiltration on CHOP protein from a canine model of multiple organ dysfunction syndrome

Objective To observe the effect of high volume hemofiltration (HVHF) on the expression of CCAAT enhancer binding protein(CHOP) during the treatment of multiple organ dysfunction syndrome (MODS). To investigate the role of CHOP protein act in apoptosis pathway mediated by the endoplasmic reticulum stress. Methods Twelve Beagle dogs were subjected to hemorrhagic shock plus resuscltation and endotixemia to establish MODS model, then they were randomly divided into two groups: HVHF group (n=6) and MODS group (n=6). After endotoxin injection completed, the HVHF group received HVHF treatment for 24 hours; MODS group did not receive. Vivo experiments: Blood samples were obtained at different time points(before operation, 0 h, 6 h, 12 h, 24 h after the injection of endotoxin). The dogs were killed and the tissue samples from lung, liver and kidney were took, then the expression of CHOP mRNA was determined. Vitro experiments: human umbilical vein endothelial cells (HUVECs) were induced by two groups’ blood samples to establish the apoptosis model. Gene expression, protein quantification and cell apoptosis rate were determined before and after the interference. Results Vivo experiments: The levels of CHOP mRNA from lung, liver and kidney had no significant difference between the two groups (P＞0.05). Vitro experiments: (1)The expression of CHOP mRNA: Compared with MODS group, the expression levels of CHOP mRNA were significantly decreased in HVHF group at 6 h, 24 h after the injection of endotoxin (P＜0.05). Compared with before, the expression levels of CHOP mRNA in the two groups were both significantly decreased after CHOP siRNA interference (P＜0.05). (2)The expression of CHOP protein: Compared with MODS group, the expression levels of CHOP protein were significantly decreased in HVHF group at each time points (P＜0.05). Compared with before, the expression levels of CHOP protein in the two groups were both significantly decreased after CHOP siRNA interference(P＜0.05). (3)Endothelial cell apoptosis rate: Compared with the preoperative rate, the two group’s endothelial cell apoptosis rate was decreased significantly at each time points(P＜0.05). Compared with MODS group, the endothelial cell apoptosis rate was significantly decreased in HVHF group at each time points(P＜0.05). Compared with before, the endothelial cell apoptosis rate in the two groups was both significantly decreased after CHOP siRNA interference(P＜0.05). Conclusion In the treatment of MODS process, HVHF can reduce endothelial cell apoptosis which may be related to the inhibition of CHOP mRNA expression and protein synthesis.